Further development of Axisymmetric Drop Shape Analysis-Captive Bubble for pulmonary surfactant related studies

The methodology combining Axisymmetric Drop Shape Analysis (ADSA) with a captive bubble (ADSA-CB) facilitates pulmonary surfactant related studies. The accuracy of ADSA-CB is crucially dependent on the quality of the bubble profile extracted from the raw image. In a previous paper, an image analysis...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2004-11, Vol.1675 (1), p.12-20
Main Authors: Zuo, Y.Y., Ding, M., Li, D., Neumann, A.W.
Format: Article
Language:English
Subjects:
Adsorption
Animals
Axisymmetric Drop Shape Analysis (ADSA)
Captive Bubble (CB)
Cattle
Dynamic surface tension measurement
Image analysis
Image Processing, Computer-Assisted
Phospholipids - metabolism
Pulmonary surfactant
Pulmonary Surfactants - chemistry
Surface Tension
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Summary:The methodology combining Axisymmetric Drop Shape Analysis (ADSA) with a captive bubble (ADSA-CB) facilitates pulmonary surfactant related studies. The accuracy of ADSA-CB is crucially dependent on the quality of the bubble profile extracted from the raw image. In a previous paper, an image analysis scheme featuring a Canny edge detector and a Axisymmetric Liquid Fluid Interfaces-Smoothing (ALFI-S) algorithm was developed to process captive bubble images under a variety of conditions, including images with extensive noise and/or lack of contrast. A new version of ADSA-CB based on that image analysis scheme is developed and applied to pulmonary surfactant and pulmonary surfactant–polymer systems. The new version is found to be highly noise-resistant and well self-adjusting.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2004.08.003