Using giant unilamellar lipid vesicle micro-patterns as ultrasmall reaction containers to observe reversible ATP synthesis/hydrolysis of F0F1-ATPase directly

F(0)F(1)-ATPase within chromatophores, which was labeled with pH-sensitive quantum dots, was encapsulated in large unilamellar lipid vesicles (LUVs) through reverse-phase evaporation. Then a microarray of chromatophore-containing LUVs was created using a micro-contact printing (mu-CP) technique. Thr...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2007-12, Vol.1770 (12), p.1620-1626
Main Authors: Liu, Xiaolong, Zhao, Rui, Zhang, Yun, Jiang, Xingyu, Yue, Jiachang, Jiang, Peidong, Zhang, Zhenxi
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - biosynthesis
Hydrogen-Ion Concentration
Hydrolysis
Lipid Metabolism
Proton-Translocating ATPases - metabolism
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Summary:F(0)F(1)-ATPase within chromatophores, which was labeled with pH-sensitive quantum dots, was encapsulated in large unilamellar lipid vesicles (LUVs) through reverse-phase evaporation. Then a microarray of chromatophore-containing LUVs was created using a micro-contact printing (mu-CP) technique. Through controlled dehydration-rehydration of the lipid patterns, a microarray of single chromatophore-containing giant unilamellar lipid vesicles (GUVs) was formed with desired size and uniform shape. The reversible ATP synthesis/hydrolysis of F(0)F(1)-ATPase in GUVs was directly observed by fluorescence microscopy through the fluorescence intensity increase/decrease in the pH-sensitive quantum dots labeled on the outer surface of the chromatophore. To the best of our knowledge, this is the first direct observation of the reversible behavior of F(0)F(1)-ATPase at the bulk scale.
ISSN:0006-3002
0304-4165
DOI:10.1016/j.bbagen.2007.08.001