Lipid fingerprints of intact viruses by MALDI-TOF/mass spectrometry

A number of viruses contain lipid membranes, which are in close contact with capsid proteins and/or nucleic acids and have an important role in the viral infection process. In this study membrane lipids of intact viruses have been analysed by MALDI-TOF/MS with a novel methodology avoiding lipid extr...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2013-04, Vol.1831 (4), p.872-879
Main Authors: Vitale, R., Roine, E., Bamford, D.H., Corcelli, A.
Format: Article
Language:English
Subjects:
Archaeal virus
Bacteriophage
coat proteins
Enveloped virus
fractionation
humans
lipid composition
lipids
Lipids - analysis
Lipids - chemistry
Mass spectrometry
Membrane lipid
Models, Biological
nucleic acids
screening
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
virion
viruses
Viruses - chemistry
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Summary:A number of viruses contain lipid membranes, which are in close contact with capsid proteins and/or nucleic acids and have an important role in the viral infection process. In this study membrane lipids of intact viruses have been analysed by MALDI-TOF/MS with a novel methodology avoiding lipid extraction and separation steps. To validate the novel method, a wide screening of viral lipids has been performed analysing highly purified intact bacterial and archaeal viruses displaying different virion architectures. Lipid profiles reported here contain all lipids previously detected by mass spectrometry analyses of virus lipid extracts. Novel details on the membrane lipid composition of selected viruses have also been obtained. In addition we show that this technique allows the study of lipid distribution easily in subviral particles during virus fractionation. The possibility to reliably analyse minute amounts of intact viruses by mass spectrometry opens new perspectives in analytical and functional lipid studies on a wider range of viruses including pathogenic human ones, which are difficult to purify in large amounts. [Display omitted] ► A new technique for the analysis of viral lipidome is described. ► Intact bacterial and archaeal viruses have been analysed by MALDI-TOF/MS. ► Minute amounts of viruses (approximately 1×107pfu) can be reliably analysed. ► Novel lipid components have been identified in selected viruses. ► Subviral fractions can also be analysed with the new technique.
ISSN:1388-1981
0006-3002
1879-2618
DOI:10.1016/j.bbalip.2013.01.011