Influence of dietary fatty acids on differentiation of human stromal vascular fraction preadipocytes

• Published in: Biochimica et biophysica acta 2015-09, Vol.1851 (9), p.1146-1155
• Main Authors: Polus, Anna, Kiec-Wilk, Beata, Razny, Urszula, Gielicz, Anna, Schmitz, Gerd, Dembinska-Kiec, Aldona
• Format: Article
• Language: English
• Subjects: Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis; Adult; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Arachidonic Acid - pharmacology; Cell Differentiation - drug effects; Cytokines - genetics; Cytokines - metabolism; Eicosanoic Acids - pharmacology; Eicosanoids; Endothelial Cells - drug effects; Endothelial Cells - metabolism; EPA; Gene Expression Profiling; Gene Expression Regulation; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Humans; Lipid Droplets - metabolism; Lipid Metabolism - drug effects; Lipidomics; Metallothionein - genetics; Metallothionein - metabolism; Microarray; Middle Aged; Monoamine Oxidase - genetics; Monoamine Oxidase - metabolism; Perilipin-3; Phospholipases A2 - genetics; Phospholipases A2 - metabolism; Primary Cell Culture; Signal Transduction; Subcutaneous Fat - drug effects; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; SVF; Vesicular Transport Proteins - genetics; Vesicular Transport Proteins - metabolism
• ISSN: 1388-1981; 0006-3002; 1879-2618
• DOI: 10.1016/j.bbalip.2015.05.002

Mediators such as cytokines, eicosanoids, nitric oxide and growth factors may regulate adipogenesis as well as inflammation. It is well documented that production of some form of eicosanoids activates lipid synthesis during adipogenesis but also contributes to the formation of factors maintaining low-level systemic inflammation. Developing nutrients for reduction of adipogenesis and inflammation can enhance preventive efficacy of daily diet. This study examined the effects of free fatty acid influence on changes in lipid biosynthesis and corresponding gene expression during differentiation of human subcutaneous adipose tissue stromal vascular fraction (SVF) cells. Proadipogenic conditions promoted SVF cell differentiation and lipid droplet (LD) formation up to 15days. This correlated with gene expression of adipocyte differentiation markers as well as inflammatory cytokines and their receptors. Addition of free fatty acids to differentiation medium increased their incorporation during the first period of differentiation (48h). Presence of eicosanoid acid (EPA) during the initial period of differentiation by elevation of Perilipin 3 protein (TIP47), may be responsible for smaller LD formation. Presence of arachidonic acid (AA) tends to deposit lipids in large form of LDs. Prolongation of differentiation up to 15days decreased AA or EPA in cellular lipids. PUFA through up-regulation of both phospholipase 2 and enzymes related to eicosanoid production influenced type and quantity of eicosanoids which regulated the extent of SVF cell differentiation. Formation of small LDs and reduction of pro-inflammatory mediators in adipose tissue are the consequence of eicosanoid production with anti-inflammatory potential from EPA. •LD formation correlated with expression of adipocyte differentiation markers.•Addition of FFAs during differentiation resulted in increasing its incorporation.•Differentiation up to 15days decreased AA and EPA amount in cellular lipids.•EPA through increasing TIP47 protein expression leads to small LD formation.•Inflammation reduction is a result of eicosanoid production from EPA.