Shuttling components of nuclear import machinery involved in nuclear translocation of steroid receptors exit nucleus via exportin-1/CRM-1 independent pathway

• Published in: Biochimica et biophysica acta 2004-05, Vol.1691 (2), p.73-77
• Main Authors: Kumar, Sanjay, Chaturvedi, Nagendra K., Nishi, Mayumi, Kawata, Mitsuhiro, Tyagi, Rakesh K.
• Format: Article
• Language: English
• Subjects: Active Transport, Cell Nucleus - physiology; alpha Karyopherins - genetics; alpha Karyopherins - metabolism; Animals; beta Karyopherins - genetics; beta Karyopherins - metabolism; Cell Nucleus - metabolism; Chlorocebus aethiops; COS Cells; Exportin 1 Protein; Fatty Acids, Unsaturated - metabolism; Karyopherins - metabolism; Leptomycin; NF-kappa B - genetics; NF-kappa B - metabolism; Nuclear export; Nuclear import; ran GTP-Binding Protein - genetics; ran GTP-Binding Protein - metabolism; Receptors, Cytoplasmic and Nuclear; Receptors, Glucocorticoid - genetics; Receptors, Glucocorticoid - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Steroid receptor; Transcription Factor RelA
• ISSN: 0167-4889; 0006-3002; 1879-2596
• DOI: 10.1016/j.bbamcr.2004.03.003

The nucleocytoplasmic transport processes are mediated by soluble transport factors constantly navigating between nuclear and cytoplasmic compartments. Our understanding about nuclear export of general ‘nuclear import factors’ that deliver the cargo to the nucleus is still fragmentary. Utilizing green fluorescent protein tagged glucocorticoid receptor (GR) and relA as our working model and with judicious use of LMB, we show in living cells that all the soluble components of the nuclear import machinery exit nucleus via exportin1/CRM1 independent pathway(s).