Isoforms of Pax5 and co-regulation of T- and B-cells associated genes influence phenotypic traits of ascetic cells causing Dalton's lymphoma

• Published in: Biochimica et biophysica acta 2011-12, Vol.1813 (12), p.2071-2078
• Main Authors: Bharti, Brij, Mishra, Rajnikant
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD19 - genetics; Antigens, CD19 - metabolism; Ascites - metabolism; Ascites - pathology; B-cells; B-Lymphocytes - metabolism; B-Lymphocytes - pathology; Blotting, Western; CD3 Complex - genetics; CD3 Complex - metabolism; Cell Proliferation; Dalton's ascite cells; Lymphoma; Lymphoma - genetics; Lymphoma - metabolism; Lymphoma - pathology; Mice; Pax5; PAX5 Transcription Factor - genetics; PAX5 Transcription Factor - metabolism; Phenotype; Protein Isoforms; raf Kinases - genetics; raf Kinases - metabolism; ras Proteins - genetics; ras Proteins - metabolism; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; T-cells; T-Lymphocytes - metabolism; T-Lymphocytes - pathology; Tumor Cells, Cultured
• ISSN: 0167-4889; 0006-3002; 1879-2596
• DOI: 10.1016/j.bbamcr.2011.08.003

The Pax5 and its isoforms influence proliferation of B- and T-cells, during development and oncogenesis but molecular mechanism and host–tumor relationship is not clear. This report describes status of Pax5 isoforms and co-regulation of molecular markers of ascite cells causing Dalton's lymphoma in murine. Higher expressions of Pax5, CD19, CD3, Ras and Raf were observed in DLA cells. The levels of transcripts as well as p53 protein were also higher in DLA cells. The transcript of p53 from DLA cells was a variant of p53 having deletion of 50bp as compared to control. On annotation, it reflects transformation related protein p53 pseudogene mRNA. Lower level of superoxide dismutase (SOD) indicates oxidative stress and higher level of LDH5 in DLA cells reflects hypoxia in cancerous condition. The expression of Pax5d/e isoforms in DLA cells suggests presence of resting B-cells. Thus, isoforms of Pax5 and co-regulation of T- and B-cells associated genes influence phenotypic traits of ascetic cells causing Dalton's lymphoma. ► Pax5 isoforms are expressed in Dalton's lymphoma ascite (DLA) cells. ► Co-regulation of T- and B-cells associated genes influence phenotypic traits of ascetic cells causing Dalton's lymphoma. ► Transcript of p53 from DLA cells represents a variant of p53.