Intracellular ion changes induced by the exposure to beta-amyloid can be explained by the formation of channels in the lysosomal membranes

• Published in: Biochimica et biophysica acta. Molecular cell research 2022-01, Vol.1869 (1), p.119145, Article 119145
• Main Authors: Zaretsky, Dmitry V., Zaretskaia, Maria V.
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - metabolism; Amyloid beta-Peptides - metabolism; Amyloid beta-Peptides - toxicity; Animals; Beta-amyloid toxicity; Calcium homeostasis; Humans; Intracellular ion disturbances; Intracellular Membranes - metabolism; Intracellular pH; Ion Transport; Lysosome; Lysosomes - metabolism; Membrane channel; Proteolytic digestion
• ISSN: 0167-4889; 1879-2596
• DOI: 10.1016/j.bbamcr.2021.119145

In this manuscript, we reassess the data on beta-amyloid-induced changes of intracellular ions concentrations published previously by Abramov et al. (2003, 2004). Their observations made using high-resolution confocal microscopy with fast temporal resolution of images formed by fluorescent ion-sensitive fluorescent probes in living cells represent an unequivocal support for the amyloid channel theory. However, closer look reveals multiple facts which cannot be explained by channel formation in plasma membrane. Recently proposed amyloid degradation toxicity hypothesis provides the interpretation to these facts by considering that channels are formed in the lysosomal membranes. •Manuscripts of Abramov et al (2003, 2004) provide convincing proof of membrane channel formation by beta-amyloid in cells.•Changes of intracellular ion concentrations cannot be explained by the formation of amyloid channels in plasma membranes.•Lysosomal permeabilization by membrane amyloid channels can explain intracellular ion disturbances caused by beta-amyloid.