Plasmalogens protect unsaturated lipids against UV-induced oxidation in monolayer

• Published in: Biochimica et biophysica acta 2003-10, Vol.1616 (2), p.137-146
• Main Authors: Morandat, Sandrine, Bortolato, Muriel, Anker, Geneviève, Doutheau, Alain, Lagarde, Michel, Chauvet, Jean-Paul, Roux, Bernard
• Format: Article
• Language: English
• Subjects: Antioxidant; Antioxidants - pharmacology; Lipid Metabolism; Lipids - radiation effects; Monolayer; Oxidation-Reduction; Phosphatidylcholines - metabolism; Plasmalogen; Plasmalogens - pharmacology; Radiation-Protective Agents - pharmacology; Ultraviolet Rays; Unsaturated lipid oxidation
• ISSN: 0005-2736; 0006-3002; 1879-2642
• DOI: 10.1016/j.bbamem.2003.08.001

Oxidative stress results from the attack by free radicals of several cellular targets (proteins, DNA and lipids). The cell equilibrium is a direct consequence of the pro-/antioxidant balance. In order to understand the physiological processes involved in oxidative stress, we followed oxidation of unsaturated lipids using a biomimetic system: Langmuir monolayers. The oxidation mode chosen was UV-irradiation and the lipid model was a polyunsaturated phospholipid: 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC). The monomolecular film technique was used to measure membrane rheology before and after UV-irradiation. We showed that the UV-irradiation of a DLPC monomolecular film led to a molecular area and surface elasticity modulus decrease that attests to the apparition of new molecular species at the air–water interface. The antioxidant effect of a synthetic plasmalogen (1-O-(1′-(Z)-hexadecenyl)-2-O-oleoyl-sn-glycero-3-phosphocholine or PPLMOPE) was tested on the oxidation of DLPC. Indeed, for about 25% mol PPLMOPE in mixed DLPC/PPLMOPE monolayers, a complete inhibition of the molecular area and the surface elasticity modulus decreases was observed in our experimental conditions. Lower PPLMOPE quantities delayed but did not prevent the DLPC oxidation in mixed monolayers.