Thermostability of Rhodopseudomonas viridis and Rhodospirillum rubrum chromatophores reflecting physiological conditions

Relationships between growth conditions and thermostability were examined for photosynthetic inner membranes (chromatophores) from Rhodopseudomonas viridis and Rhodospirillum rubrum of which morphology, lipid composition, and protein/lipid rate are rather mutually different. Signals observed by diff...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2011-06, Vol.1808 (6), p.1645-1653
Main Authors: Odahara, Takayuki, Ishii, Noriyuki, Ooishi, Ayako, Honda, Shinya, Uedaira, Hatsuho, Hara, Masayuki, Miyake, Jun
Format: Article
Language:English
Subjects:
Bacterial Chromatophores - chemistry
Bacterial Chromatophores - ultrastructure
Calorimetry, Differential Scanning
Cholic Acids - chemistry
Chromatophore
Circular Dichroism
Differential scanning calorimetry
Microscopy, Electron
Photoreaction unit
Photosynthetic bacterium
Photosynthetic Reaction Center Complex Proteins - chemistry
Photosynthetic Reaction Center Complex Proteins - metabolism
Protein Stability
Protein Unfolding
Rhodopseudomonas - chemistry
Rhodopseudomonas - growth & development
Rhodospirillum rubrum - chemistry
Rhodospirillum rubrum - growth & development
Spectrophotometry
Temperature
Thermostability
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Summary:Relationships between growth conditions and thermostability were examined for photosynthetic inner membranes (chromatophores) from Rhodopseudomonas viridis and Rhodospirillum rubrum of which morphology, lipid composition, and protein/lipid rate are rather mutually different. Signals observed by differential scanning calorimetry of the chromatophores were correlated with thermal state transitions of the membrane components by reference to temperature dependencies of circular dichroism and absorption spectra of the purified supramolecule comprising a photoreaction center and surrounding light-harvesting pigment–protein complexes that are the prominent proteins in both membranes. The differential scanning calorimetry curves of those chromatophores exhibited different dependencies on growth stages and environmental temperatures. The obtained result appeared to reflect the differences in the protein/lipid rate and protein–lipid specificity between the two chromatophores. ►The dynamics of intact membranes is fundamental for understanding the functions. ►Effect of growth conditions on between dynamics was examined for two chromatophores. ►DSC of the two membranes exhibited different temperature dependence. ►The difference was evaluated well in terms of protein–lipid rate and specificity.
ISSN:0005-2736
0006-3002
1879-2642
DOI:10.1016/j.bbamem.2011.02.013