Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin

Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxi...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2016-01, Vol.1858 (1), p.139-145
Main Authors: Thomas, Andrés H., Catalá, Ángel, Vignoni, Mariana
Format: Article
Language:English
Subjects:
Glycine max - chemistry
Lipid Peroxidation - radiation effects
Lipid Peroxides - chemistry
Liposomes - chemistry
Liposomes - radiation effects
Permeability
Phosphatidylcholines - chemistry
Photosensitizing Agents - chemistry
Pterins - chemistry
Ultraviolet Rays
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Summary:Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.
ISSN:0005-2736
0006-3002
1879-2642
DOI:10.1016/j.bbamem.2015.11.002