Pentobarbital modifies the lipid raft-protein interaction: A first clue about the anesthesia mechanism on NMDA and GABA A receptors

Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta 2016-11, Vol.1858 (11), p.2603-2610
Main Authors: Sierra-Valdez, Francisco Javier, Ruiz-Suárez, J C, Delint-Ramirez, Ilse
Format: Article
Language:English
Subjects:
Anesthesia
Animals
Brain - drug effects
Brain - metabolism
Gene Expression
Hypnotics and Sedatives - metabolism
Hypnotics and Sedatives - pharmacology
Male
Membrane Microdomains - chemistry
Membrane Microdomains - drug effects
Membrane Microdomains - metabolism
Pentobarbital - metabolism
Pentobarbital - pharmacology
Rats
Rats, Wistar
Receptors, GABA-A - biosynthesis
Receptors, GABA-A - genetics
Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate - biosynthesis
Receptors, N-Methyl-D-Aspartate - genetics
Synaptosomes - chemistry
Synaptosomes - drug effects
Synaptosomes - metabolism
Transition Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABA receptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.
ISSN:0006-3002
0005-2736
DOI:10.1016/j.bbamem.2016.07.011