Effect of cholesterol on the hydration properties of ester and ether lipid membrane interphases

Fluorescence spectroscopy and Molecular Dynamics results show that cholesterol reduces water along the chains in ether lipids by changing the water distribution pattern between tightly and loosely bound water molecules. Water distribution was followed by emission spectra and generalized polarization...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Biomembranes 2021-01, Vol.1863 (1), p.183489, Article 183489
Main Authors: Pérez, H.A., Alarcón, L.M., Verde, A.R., Appignanesi, G.A., Giménez, R.E., Disalvo, E.A., Frías, M.A.
Format: Article
Language:English
Subjects:
14:0 Diether PC
Cholesterol
Cholesterol - chemistry
DMPC
Generalized polarization
Hydration
Lipid Bilayers - chemistry
Molecular Dynamics
Phosphatidylcholines - chemistry
Spectrometry, Fluorescence
Structure-Activity Relationship
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Summary:Fluorescence spectroscopy and Molecular Dynamics results show that cholesterol reduces water along the chains in ether lipids by changing the water distribution pattern between tightly and loosely bound water molecules. Water distribution was followed by emission spectra and generalized polarization of 6-dodecanoyl-2-dimethyl aminonaphthalene (Laurdan) inserted in 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine (14: 0 Diether PC) membranes. Molecular Dynamics simulations indicate that the action of cholesterol could be different in ether PC in comparison to ester PC. In addition, Cholesterol seems to act “per se” as an additional hydration center in ether lipids. Regardless of the phase state, cholesterol both in DMPC and 14:0 Diether PC vesicles, changed the distribution of water molecules decreasing the dipole relaxation of the lipid interphase generating an increase in the non-relaxable population. Above 10% Cholesterol/14:0 Diether PC ratio vesicles' interphase present an environment around Laurdan molecules similar to that corresponding to ester PC. [Display omitted] •Cholesterol conforms a new hydration site in membranes lacking carbonyl groups.•An increase in 14:0 Diether PC polarization is produced at 10% Chol below Tm not observed in ester PC.•The transition observed at 10% Chol in 14:0 Diether PC is due to an increase of non relaxable water molecules population.
ISSN:0005-2736
1879-2642
DOI:10.1016/j.bbamem.2020.183489