Pleiotropic effects of a cold shock protein homolog PprM on the proteome of Deinococcus radiodurans

• Published in: Biochimica et biophysica acta. Proteins and proteomics 2019-02, Vol.1867 (2), p.98-106
• Main Authors: Anaganti, Narasimha, Padwal, Mahesh Kumar, Dani, Pratiksha, Basu, Bhakti
• Format: Article
• Language: English
• Subjects: Deinococcus radiodurans; Gamma-radiation; Gyrase B; Housekeeping pathways; PprM
• ISSN: 1570-9639; 1878-1454
• DOI: 10.1016/j.bbapap.2018.10.015

An extremophile D. radiodurans encodes a non-cold shock inducible cold shock protein homolog DR_0907 (also known as PprM). The DR_0907 ORF was deleted by knockout mutagenesis and the resultant deletion mutant (ΔpprM D. radiodurans) displayed growth defect as well as gamma-radiation sensitivity (D10 values = ΔpprM D. radiodurans: 12.1 kGy versus wild type (WT) D. radiodurans: 14 kGy). 2D gel based comparative proteomics revealed a comparable induction of DNA repair proteins in ΔpprM D. radiodurans and WT D. radiodurans recovering from 5 kGy gamma irradiation (60Co gamma source, dose rate: 2 kGy/h), suggesting that pprM does not cause radiation sensitivity through modulation of DdrO-regulated DNA repair genes. However, deletion of pprM did result in repression of several proteins that belonged to vital housekeeping pathways such as metabolism and protein homeostasis that might contribute to slow growth phenotype. These deficiencies intrinsic to ΔpprM D. radiodurans might also contribute to its radiation sensitivity. [Display omitted] •DR_0907 from D. radiodurans encodes a cold-shock protein homolog PprM.•DR_0907 knockout mutant is defective in growth and sensitive to gamma radiation.•Absence of PprM does not affect induction of DdrO-regulated DNA repair proteins.•Absence of PprM results in modulation of several house-keeping pathways.•Multi-stress sensitivity of mutant ascribed to deficient house-keeping functions.