42. The spatial energy expenditure configuration and possible applications in an experimental model of arthritis

• Published in: Brain, behavior, and immunity behavior, and immunity, 2013-09, Vol.32, p.e12-e12
• Main Authors: Klatt, S, Straub, R.H
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Psychiatry
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2013.07.054

Background : Autoimmune responses with differentiation and proliferation of immune cells and the subsequent tissue-directed inflammatory process in the symptomatic phase of the disease are very energy-demanding. We hypothesize that during development of arthritis reallocation of energy-rich fuels to the activated immune system is necessary in order to nourish the immunological and inflammatory process. Energy consumption and ATP generation can be measured by studying oxygen consumption. Methods : A new technique termed “spatial energy expenditure configuration” (SEEC) is based on removal of tissue during the course of arthritis, and subsequent determination of oxygen consumption in vitro. Results : In draining lymph nodes of arthritic DBA/1J mice, we observed a marked increase in oxygen consumption during the course of arthritis. Sympathectomy prior to immunization increases energy consumption in lymph nodes, which is most probably a sign of retention of leucocytes in the lymph node. C57BL/6 mice deficient for the important adipose triglyceride lipase revealed increased oxygen consumption in the liver. This might be due to a lack of lipolysis activity and, therefore, increased gluconeogenetic activity in the liver for the generation of glucose. ATGL-deficient arthritic animals also showed higher energy demand in lymph nodes, adrenal glands, and gut. Conclusions : The SEEC technique enables us to identify locations of high energy demand that are involved in the initiation and continuation of the autoimmune process in an animal model of arthritis.