Pivotal participation of nitrogen dioxide in l-arginine induced acute necrotizing pancreatitis: protective role of superoxide scavenger 4-OH-TEMPO

For the first time, a direct sensitive method of NO 2 detection and measurement in biological material has been established. It is based on the interaction of this radical with the coordination compound of Cr(III) with aminodeoxysugar as biosensor. Our new method makes it possible to precisely asses...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-01, Vol.326 (2), p.313-320
Main Authors: Dąbrowska, Aleksandra, Jacewicz, Dagmara, Łapińska, Agnieszka, Banecki, Bogdan, Figarski, Adam, Szkatuła, Michał, Lehman, Jędrzej, Krajewski, Jacek, Kubasik-Juraniec, Jolanta, Woźniak, Michał, Chmurzyński, Lech
Format: Article
Language:English
Subjects:
4-OH-TEMPO
Acute pancreatitis
Aminodeoxysugars
Amylases - blood
Animals
Arginine - pharmacology
Chromium(III) complexes
Cyclic N-Oxides - pharmacology
Cytosol - metabolism
Free Radical Scavengers - pharmacology
Kinetics
l-Arginine
Male
Mechanism
Microscopy, Electron
Molecular Structure
Nitrogen dioxide
Nitrogen Dioxide - analysis
Nitrogen Dioxide - chemistry
Nitrogen Dioxide - metabolism
NO 2
Pancreatitis, Acute Necrotizing - chemically induced
Pancreatitis, Acute Necrotizing - metabolism
Pancreatitis, Acute Necrotizing - pathology
Pancreatitis, Acute Necrotizing - prevention & control
Rats
Rats, Wistar
Spectrum Analysis
Spin Labels
Sulfhydryl Compounds - metabolism
Superoxide
Superoxides - metabolism
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Summary:For the first time, a direct sensitive method of NO 2 detection and measurement in biological material has been established. It is based on the interaction of this radical with the coordination compound of Cr(III) with aminodeoxysugar as biosensor. Our new method makes it possible to precisely assess NO 2 level in experimental acute necrotizing pancreatitis induced by l-arginine, where oxidative and nitrosative stresses are supposed to play a key role in the pathomechanism of the disease. As much as 20 nmol of NO 2/mg protein was detected which correlated with severe deterioration of pancreatic acinar cell ultrastructure. Protective effect of superoxide radical scavenger 4-OH-TEMPO expressed as NO 2 level decrease confirmed by preserved acinar cell ultrastructure and decreased pancreatic amylase release to blood serum is demonstrated. This study reveals a possible pathomechanism of l-arginine induced acute pancreatitis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.11.032