Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2

It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear pa...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-05, Vol.369 (3), p.884-888
Main Authors: Chang, Chih-Hung, Fang, Hsu-Wei, Ho, Yi-Ching, Huang, Huei-Ting
Format: Article
Language:English
Subjects:
Animals
Arthroplasty, Replacement - adverse effects
Cartilage, Articular - cytology
Cartilage, Articular - immunology
Cartilage, Articular - surgery
Chondrocytes - immunology
Chondrocytes - ultrastructure
Coculture Techniques
Dinoprostone - metabolism
Flow Cytometry
Microscopy, Electron, Transmission
Nitric Oxide - metabolism
Osteoarthritis - immunology
Phagocytes - immunology
Phagocytosis
Polyethylenes - adverse effects
Polyethylenes - metabolism
Sus scrofa
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Summary:It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE(2). In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.02.123