β1 integrins regulate chondrogenesis and rock signaling in adipose stem cells

β1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell–cell to cell–matrix interactions, we hypothesized that β1 integrins play a different role at the earlier (mainly cell–cell interaction) from the later stage (main...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-08, Vol.372 (4), p.547-552
Main Authors: Lu, Z.F., Doulabi, B. Zandieh, Huang, C.L., Bank, R.A., Helder, M.N.
Format: Article
Language:English
Subjects:
Adipose tissue
Cell shape
Chondrogenesis
Mesenchymal stem cells
Rock signaling
β1 integrins
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Summary:β1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell–cell to cell–matrix interactions, we hypothesized that β1 integrins play a different role at the earlier (mainly cell–cell interaction) from the later stage (mainly cell–matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by β1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both β1-integrin blockage and TGF-β1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that β1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by β1-integrin blockage might be through inhibiting Rock signaling.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.05.063