Involvement of TRPC3 channels in calcium oscillations mediated by OX1 orexin receptors

Oscillations of intracellular Ca2+ provide a novel mechanism for sustained activation of cellular processes. Receptor-activated oscillations are mainly thought to occur through rhythmic IP3-dependent store discharge. However, as shown here in HEK293 cells 1nM orexin-A (Ox-A) acting at OX1 receptors...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-07, Vol.385 (3), p.408-412
Main Authors: Peltonen, Hanna M., Magga, Johanna M., Bart, Genevieve, Turunen, Pauli M., Antikainen, Miia S.H., Kukkonen, Jyrki P., Åkerman, Karl E.
Format: Article
Language:English
Subjects:
Calcium oscillations
Hypocretin-1
Orexin-A
OX1 receptor
Phospholipase A2
Receptor
TRP channel
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Summary:Oscillations of intracellular Ca2+ provide a novel mechanism for sustained activation of cellular processes. Receptor-activated oscillations are mainly thought to occur through rhythmic IP3-dependent store discharge. However, as shown here in HEK293 cells 1nM orexin-A (Ox-A) acting at OX1 receptors (OX1R) triggered oscillatory Ca2+ responses, requiring external Ca2+. These responses were attenuated by interference with TRPC3 channel (but not TRPC1/4) function using dominant negative constructs, elevated Mg2+ (a blocker of many TRP channels) or inhibition of phospholipase A2. These treatments did not affect Ca2+ oscillations elicited by high concentrations of Ox-A (100nM) in the absence of external Ca2+. OX1R are thus able to activate TRPC(3)-channel-dependent oscillatory responses independently of store discharge.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.05.077