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A mouse model of weight gain after nicotine withdrawal

Smoking cessation increases body weight. The underlying mechanisms, however, have not been fully understood. We here report an establishment of a mouse model that exhibits an augmented body weight gain after nicotine withdrawal. High fat diet-fed mice were infused with nicotine for two weeks, and th...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2022-01, Vol.588, p.140-146
Main Authors: Takeda, Katsushi, Aotani, Daisuke, Kuga, Yusuke, Jinno, Tomoki, Guo, Tingting, Ogawa, Kento, Shimizu, Yuki, Hattori, Rei, Yagi, Takashi, Koyama, Hiroyuki, Matsumura, Shigenobu, Kataoka, Hiromi, Tanaka, Tomohiro
Format: Article
Language:English
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Summary:Smoking cessation increases body weight. The underlying mechanisms, however, have not been fully understood. We here report an establishment of a mouse model that exhibits an augmented body weight gain after nicotine withdrawal. High fat diet-fed mice were infused with nicotine for two weeks, and then with vehicle for another two weeks using osmotic minipumps. Body weight increased immediately after nicotine cessation and was significantly higher than that of mice continued on nicotine. Mice switched to vehicle consumed more food than nicotine-continued mice during the first week of cessation, while oxygen consumption was comparable. Elevated expression of orexigenic agouti-related peptide was observed in the hypothalamic appetite center. Pair-feeding experiment revealed that the accelerated weight gain after nicotine withdrawal is explained by enhanced energy intake. As a showcase of an efficacy of pharmacologic intervention, exendin-4 was administered and showed a potent suppression of energy intake and weight gain in mice withdrawn from nicotine. Our current model provides a unique platform for the investigation of the changes of energy regulation after smoking cessation. •Smoking cessation is accompanied by increased body weight.•A mouse model of weight gain after nicotine withdrawal is established.•Food intake and orexigenic peptide expression are enhanced immediately after nicotine withdrawal.•Dependence of body weight gain on increased food intake is shown by pair-feeding experiment.•Exendin-4, a glucagon-like peptide-1 analog, proves to be an effective therapy.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2021.12.064