Antibacterial and antibiofilm assessment of Momordica charantia fruit extract coated silver nanoparticle

• Published in: Biocatalysis and agricultural biotechnology 2016-10, Vol.8, p.189-196
• Main Authors: Malaikozhundan, Balasubramanian, Vaseeharan, Baskaralingam, Vijayakumar, Sekar, Sudhakaran, Raja, Gobi, Narayanan, Shanthini, Ganesan
• Format: Article
• Language: English
• Subjects: Antibacterial; Antibiofilm; Momordica charantia; Silver nanoparticle; TEM
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2016.09.007

Silver nanoparticles were synthesized using the fruit extracts of Momordica charantia (Mc-AgNPs). The structural characterization of Mc-AgNPs was performed by UV–vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). UV–vis recorded the absorbance spectra at 460nm. XRD shows the crystalline nature of silver nanoparticles with various Bragg's reflection peaks at 111, 200, 220 and 311. FTIR spectra of the synthesized Mc-AgNPs showed strong bands at 1382, 1203, 1151, 1102, 1013 and 654cm−1 which corresponds to O–H, C–H, C–C, C–OH and C–N groups. TEM showed the spherical shape of Mc-AgNPs with particle size between 16nm. The antibacterial activity of Mc-AgNPs was tested against Gram-positive and Gram-negative bacteria. Mc-AgNPs showed greater inhibition of Enterococcus faecalis at 100µgml−1 compared to Aeromonas hydrophila. The biofilm inhibition of Mc-AgNPs was also higher against E. faecalis compared to A. hydrophila. [Display omitted] •Momordica charantia fruit extracts coated silver nanoparticle was synthesized.•Mc-Ag NPs were characterized by UV spectroscopy, XRD, FTIR, and TEM.•Mc-Ag NPs controlled the growth of Enterococcus faecalis at 100μgml−1.•Mc-Ag NPs inhibited the biofilm of E. faecalis at 100μgml−1.