Development of antibody anti-FimC-Salmonella typhi as a detection kit model of typhoid diseases by antigen capture approach

• Published in: Biocatalysis and agricultural biotechnology 2019-05, Vol.19, p.101157, Article 101157
• Main Authors: Nurjayadi, Muktiningsih, Kurnia Dewi, Fera, Kartika, Irma Ratna, Hasan, Umar, Setianingsih, Ida, asiah, Nur, Wiguna, Delia Ayu, Marcella, Anis, Puspasari, Fernita, Sulfianti, Asri, Agustini, Kurnia, Wardoyo, Wibowo Mangun, El-Enshasy, Hesham Ali
• Format: Article
• Language: English
• Subjects: Anti-fim-C-S. typhi antibodies; Prototype detection tool; Typhoid diseases
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2019.101157

Typhoid fever is a world health problem, with 200,000 recorded death annually in developing countries. The discovery of new drug discovery and detection methods for typhoid is still continuing. In previous research, the 31 kilo Dalton (kDa) recombinant protein Fim-C-S typhi was successfully expressed. It was also reported that the recombinant protein Fim-C-S. typhi could induce the occurrence of antibodies. This study aims to further develop anti-Fim-C-S. typhi antibodies as a detection tool. The sensitivity evaluated by western immunoblotting analysis indicated that anti-Fim-C-S. typhi antibodies can significantly recognize its antigen at a minimum level of 0.125 μg. The specificity evaluation of anti-FimC-S. typhi antibodies against S. typhi bacteria extract protein showed that anti-Fim-C-S. typhi antibodies could recognize S. typhi extract protein at ± 29 KDa and ±60 kDa. In addition, anti-Fim-C-S. typhi antibody did not recognize healthy blood extract proteins. Simulation in healthy blood samples containing bacterial antigen S. typhi and recombinant antigen Fim-C-S. typhi produce bands of 29 kDa, 31 kDa and 60 kDa have been also studied. It can be concluded that anti-Fim-C-S. typhi antibodies can be used in the development of prototype detection tool. The results from this study are expected to provide a foundation to the development of a detection methods for S. typhi that are sensitive, specific, safe and simple. •Anti-Fim-C-S. typhi antibodies can be a prototype for the detection of S. typhi.•Anti-Fim-C-S. typhi antibodies could recognize S. typhi extract at 29 kDa and 60 kDa.•Anti-Fim-C-S. typhi antibodies did not recognize healthy blood extract proteins.•Simulation of bacterial antigen S. typhi and recombinant antigen Fim-C-S.typhi in the healthy blood produced bands at 29 kDa, 31 KDa and 60 KDa upon immunoblotting.