Liquid lipase-mediated production of biodiesel from agroindustrial waste

• Published in: Biocatalysis and agricultural biotechnology 2020-11, Vol.30, p.101864, Article 101864
• Main Authors: Cavali, Matheus, Bueno, Auriane, Fagundes, Ana P., Priamo, Wagner L., Bilibio, Denise, Mibielli, Guilherme M., Wancura, João H.C., Bender, João P., Oliveira, J. Vladimir
• Format: Article
• Language: English
• Subjects: Biodiesel; Biomass; Enzymatic solution; Esterification; Lipase; Waste recovery
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2020.101864

Decreasing in fossil fuel reserves and its adverse environmental effects motivate the development of the biofuels production, such as biodiesel. Animal fats residues, generated by agro-processing industries in large quantities, can be considered a low-cost raw material for conversion to alkyl esters (biodiesel). This study aims to evaluate the production of biodiesel (fatty acid methyl esters – FAME) from enzymatic catalysis applying the novel commercial lipase in liquid formulation NS-40116 obtained from the modified Thermomyces lanuginosus microorganism, using residual chicken oil as feedstock. The reaction parameters “oil to methanol molar ratio”, “water content” and “temperature” were investigated through a statistical design. At 200 rpm during 36 h, enzymatic catalysis (0.3 wt% of lipase to residual chicken oil) presented the most appropriate condition at 35 °C, 2 wt% of water content and oil to methanol molar ratio of 1:4, yielding 93.16% in FAME. Therefore, the recovery of that waste was satisfactorily performed in feasible conditions, producing a less environment-harmful biofuel. [Display omitted] •Low-cost residual chicken fat was proposed for enzyme-catalyzed biodiesel production.•The lipase NS-40116 from T. lanuginosus in liquid formulation was utilized.•Temperature, fat to methanol molar ratio and water content were assessed.•It was obtained 93.16% of fatty acid methyl esters (FAME) at feasible conditions.