Intrinsic modifying-antibiotic activity of a liposomal structure against MRSA and other MDR bacteria

• Published in: Biocatalysis and agricultural biotechnology 2021-01, Vol.31, p.101880, Article 101880
• Main Authors: Geraldo de Alencar Santos Júnior, José, Freitas, Priscila Ramos, Rocha, Janaína Esmeraldo, Silva Pereira, Raimundo Luiz, Datiane de Morais Oliveira – Tintino, Cícera, Gonçalves, Vanessa Pinheiro, Viana Rodrigues, João Pedro, Melo Coutinho, Henrique Douglas, Nicolete, Roberto
• Format: Article
• Language: English
• Subjects: Antibiotics; Liposomes; Nanocarriers; Nanotechnology
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2020.101880

This work aimed to develop and characterize liposomes and evaluate their antimicrobial carrier activity. In the preparation of liposomes, dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS) and cholesterol (COL) in a proportion of 5: 3: 1 were used. Vesicle diameter, Polydispersion Index and Zeta Potential analyzes were performed. Atomic Force Microscopy (MFA) and Scanning Electron Microscopy (SEM) were used to determine the shape and morphology of the liposome surface. Microbiological assays were performed against standard and multiresistant bacteria Staphylococcus aureus and Escherichia coli. For the liposome modulating effect test without incorporation with norfloxacin and gentamicin reference antibiotics, the fraction was used at a subinhibitory concentration (MIC/8) and the drugs were microdiluted. The work follows a prospective approach to develop a possible liposomal source with antimicrobial reader properties. •Synergistic effect of liposomes with antibiotics was observed.•New formulations based on lipid nanoparticles with antimicrobial properties are possible.•The extrusion technique is capable of producing liposomes with better parameters.