Multiplex molecular marker-assisted analysis of significant pathogens of cotton (Gossypium sp.)

Plant pathogens diminish crop quality and yield, leading to economic losses. Molecular markers have the potential for early and accurate detection of pathogens. DNA markers based on ITS regions, pthN gene, and CP gene were designed to detect strains of fungal, bacterial, and viral pathogens, respect...

Full description

Saved in:
Bibliographic Details
Published in:Biocatalysis and agricultural biotechnology 2023-01, Vol.47, p.102557, Article 102557
Main Authors: Chavhan, R.L., Sable, S., Narwade, A.V., Hinge, V.R., Kalbande, B.B., Mukherjee, A.K., Chakrabarty, P.K., Kadam, Ulhas S.
Format: Article
Language:English
Subjects:
CAPS
Cotton
Disease control
Molecular biodiagnostics
Molecular markers
Multiplex-PCR
Pathogen diagnosis
rDNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant pathogens diminish crop quality and yield, leading to economic losses. Molecular markers have the potential for early and accurate detection of pathogens. DNA markers based on ITS regions, pthN gene, and CP gene were designed to detect strains of fungal, bacterial, and viral pathogens, respectively. The pRS and pRB primers were found specific to strains of R. solani and R. bataticola. Four strains of R. areola isolates from each cultivated cotton species were detected using the primer pRARE. Further, CAPS markers, Bst6I, and DraRI specific to the strains of Alternaria macrospora were developed, which could differentiate from other fungal pathogens. Furthermore, we developed a multiplexed assay to detect six pathogens simultaneously. These diagnostic markers could help assess the prevalence of cotton infecting pathogens in the field (from plant tissue or soil). Consequently, the diagnostic assay could be used for undertaking judicious disease control measures in commercial agriculture. •Plant pathogens damage crop quality and yield; lead to economic losses.•Molecular markers have potential for early and accurate detection of various pathogens of cotton.•Mmarkers were designed to detect cotton's significant fungal, bacterial and viral pathogens.•Multiplexed analysis of various cotton pathogens is performed.
ISSN:1878-8181
1878-8181
DOI:10.1016/j.bcab.2022.102557