Novel peroxidase from bitter leaf (Vernonia amygdalina): Purification, biochemical characterization and biotechnological applications

• Published in: Biocatalysis and agricultural biotechnology 2023-05, Vol.49, p.102662, Article 102662
• Main Authors: Ilesanmi, Olutosin Samuel, Adedugbe, Omowumi Funke
• Format: Article
• Language: English
• Subjects: Characterization; Dye decolorization; Novel peroxidase; Purification; Reporter enzyme; Vernonia amygdalina
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2023.102662

Bitter leaf could serve as a cost-effective and alternative source of plant peroxidase for several applications. In this study, the bioavailability, biochemical properties and potentials of bitter leaf peroxidase in some biotechnological applications are described. The enzyme was first purified using a fast and cost-effective approach. The combination of aqueous two-phase partitioning and gel filtration chromatography on Bio Gel P-100 was very efficient resulting to a percentage yield and final purification fold of 76% and 7.1 respectively. Native and subunit molecular weights of the purified peroxidase were 41.2 ± 2.1 kDa and 39.5 ± 1.0 kDa, respectively, revealing the monomeric nature of bitter leaf POD. The optimum pH was 6.0 while that of temperature was 30 °C. The estimated kinetic constants Km were 2.5 ± 0.1 mM and 5.1 ± 0.2 mM for o-dianisidine and hydrogen peroxide respectively while maximum velocity (Vmax) was 3010.6 ± 21 units/mg protein. The enzyme was activated in the presence of organic solvents (at up to 30% chloroform). It also retained stability (≥60%) at up to 50% concentration of acetone, methanol and ether. Chlorides of Ca2+, Na+ and Cu2+ activated the enzyme whereas Al3+ and Fe3+ had inhibitory effects. Bitter leaf POD was covalently bonded with casein to form higher molecular adduct, establishing its potentials as a reporter enzyme. In conclusion, bitter leaf peroxidase possesses remarkable biochemical properties that could be of interest in several industrial and biotechnological applications. •Peroxidase of about 367 U/mg was extracted from bitter leaf (Vernonia amygdalina).•The bitter leaf peroxidase was purified using a fast and cost effective purification scheme.•Biochemical properties of the bitter leaf peroxidase were investigated.•Biotechnological applications of the bitter leaf peroxidase were examined.•The potential of the bitter leaf peroxidase as a reporter enzyme was also established.