Preparation of nanofibrous polymer grafted magnetic poly(GMA-MMA)-g-MAA beads for immobilization of trypsin via adsorption

Poly(glycidylmethacrylate-methylmethacrylate), poly(GMA-MMA) beads were prepared via suspension polymerization in the presence of ferric ions. The epoxy groups of the poly(GMA-MMA) beads were converted into amino groups during magnetization reaction, and then were grafted with methacrylic acid (MAA)...

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Bibliographic Details
Published in:Biochemical engineering journal 2008-06, Vol.40 (2), p.262-274
Main Authors: BAYRAMOFLU, Giilay, YILMAZ, Meltem, SENEL, Aysegül Ulkü, ARICA, M. Yakup
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biotechnology
Enzyme technology
Fundamental and applied biological sciences. Psychology
General aspects
Immobilization techniques
Magnetic beads
Methods. Procedures. Technologies
Peptide maps
Protease
Protein
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Summary:Poly(glycidylmethacrylate-methylmethacrylate), poly(GMA-MMA) beads were prepared via suspension polymerization in the presence of ferric ions. The epoxy groups of the poly(GMA-MMA) beads were converted into amino groups during magnetization reaction, and then were grafted with methacrylic acid (MAA) via graft copolymerization. The magnetic beads were characterized by surface area measurement, swelling test, scanning electron microscope (SEM), electron spin resonance (ESR) and Mössbauer spectroscopy. The enzyme “trypsin” was immobilized on the magnetic beads via adsorption. The maximum adsorption was obtained at pH 7.0. At 2.0 mg/mL initial trypsin concentration, the maximum immobilization capacity was 123.2 mg trypsin/g beads and retained about 84.2% of its initial activity. The immobilized trypsin could not be desorbed by enzyme reaction solution in the pH range of 5.0–9.0, and could be desorbed by 1.0 M formic acid solution containing 1 M NaCl.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2007.12.013