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Kinetics of enzymatic synthesis of monoferuloyl glycerol and diferuloyl glycerol by transesterification in [BMIM]PF6

[Display omitted] •Max yields of monoferuloyl glycerol (63.72±1.26%) and diferuloyl glycerol (78.80±2.09%) were achieved.•The activation energy for ethyl ferulate conversion was 40.16kJ/mol.•The activation energies for monoferuloyl glycerol and diferuloyl glycerol formations were 31.43, and 85.38kJ/...

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Bibliographic Details
Published in:Biochemical engineering journal 2015-05, Vol.97, p.25-31
Main Authors: Sun, Shangde, Chen, Xiaowei
Format: Article
Language:English
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Summary:[Display omitted] •Max yields of monoferuloyl glycerol (63.72±1.26%) and diferuloyl glycerol (78.80±2.09%) were achieved.•The activation energy for ethyl ferulate conversion was 40.16kJ/mol.•The activation energies for monoferuloyl glycerol and diferuloyl glycerol formations were 31.43, and 85.38kJ/mol, respectively.•Michaelis constants for ethyl ferulate and glycerol were 0.23 and 0.42mol/L, respectively.•Transesterification mechanism with the inhibitions of ethyl ferulate and glycerol was proposed. Feruloyl glycerols (FGs), water-soluble glycerides (monoferuloyl glycerol (MFG) and diferuloyl glycerol (DFG)) of ferulic acid, can be used as natural ultraviolet (UV) filters and antioxidants in chemical, food, pharmaceuticals, and drug industries. In order to promote the synthesis of FGs, the effect of process parameters, optimization, thermodynamic and kinetic properties on the enzymatic transesterification of ethyl ferulate (EF) with glycerol in [BMIM]PF6 were investigated. The maximum yields of MFG (63.72±1.26%) and DFG (78.80±2.09%) were achieved at low glycerol concentrations in [BMIM]PF6. The activation energies for EF conversion and transesterification to form MFG and DFG were calculated as 40.16, 31.43 and 85.38kJ/mol, respectively, based on the Arrhenius law. Reaction kinetics agreed with the Ping–Pong Bi–Bi mechanism with the inhibitions of EF and glycerol. The enzymatic mechanism of the transesterification of EF with glycerol in [BMIM]PF6 was also proposed.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2015.02.002