Exploring the production of citric acid with Yarrowia lipolytica using corn wet milling products as alternative low-cost fermentation media

• Published in: Biochemical engineering journal 2020-03, Vol.155, p.107463, Article 107463
• Main Authors: Cavallo, Ema, Nobile, Matías, Cerrutti, Patricia, Foresti, María Laura
• Format: Article
• Language: English
• Subjects: Alternative low-cost nutrient sources; Citric acid production; Corn wet milling products; Yarrowia lipolytica
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2019.107463

[Display omitted] •Production of CA with Y. lipolytica in alternative low- cost media is proposed.•Corn wet milling products such as corn syrups and CSL are evaluated.•Among the Y. lipolytica strains tested, highest CA production is obtained with W29.•HFS and CSL appear as promising nutrient sources for CA production at higher scale. The unconventional yeast Yarrowia lipolytica is known for its capability to synthesize different metabolites of industrial interest such as citric acid (CA). The numerous applications of CA trigger continuous research on improved production systems and substrates costs reduction. In this context, the present contribution explored the production of CA using chosen strains of Y. lipolytica in fermentation media formulated with low-cost and non-seasonal nutrient sources derived from the corn wet milling process, such as food grade corn syrups and corn steep liquor (CSL). Besides nutrient sources assay, the effect of carbon source concentration and C/N ratio was evaluated. In media formulated with high fructose syrup and CSL proper manipulation of the mentioned variables allowed attaining CA productions of up to 38 g/L in flasks culture. Moreover, the bioreactor scale-up allowed a 130%-increase in productivity and drastic fermentation time reduction. Overall, the combination of these low-cost nutrient sources of predictable composition derived from the corn processing industry that may be stored at room temperature seems attractive for CA production. The simplicity of the fermentation medium further makes it promising for higher-than-laboratory scale production of CA.