Kupffer cell cytokines interleukin-1β and interleukin-10 combine to inhibit phosphoenolpyruvate carboxykinase and gluconeogenesis in cultured hepatocytes

Background and aims: Recent evidence suggests that inflammatory cytokines may mediate reduced hepatic glucose production and reduced blood glucose concentrations in sepsis. Therefore the aim of this study is to provide direct evidence of a cytokine-mediated interaction between Kupffer cells and hepa...

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Bibliographic Details
Published in:The international journal of biochemistry & cell biology 2004-08, Vol.36 (8), p.1462-1472
Main Authors: Yerkovich, Stephanie T, Rigby, Paul J, Fournier, Paul A, Olynyk, John K, Yeoh, George C.T
Format: Article
Language:English
Subjects:
Cytokines
Gluconeogenesis
Kupffer cells
Phosphoenolpyruvate carboxykinase
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Summary:Background and aims: Recent evidence suggests that inflammatory cytokines may mediate reduced hepatic glucose production and reduced blood glucose concentrations in sepsis. Therefore the aim of this study is to provide direct evidence of a cytokine-mediated interaction between Kupffer cells and hepatocytes by characterising the effects of lipopolysaccharide-stimulated Kupffer cells on hepatocyte gluconeogenesis, and the activity of key regulatory enzymes of this pathway. Methods and results: Primary isolates of hepatocytes co-cultured with lipopolysaccharide-stimulated Kupffer cells in Transwell inserts showed a 48% inhibition of gluconeogenesis ( P
ISSN:1357-2725
1878-5875
DOI:10.1016/j.biocel.2003.10.022