Profiling gene expression of whole cytochrome P450 superfamily in human bronchial and peripheral lung tissues: Differential expression in non-small cell lung cancers

Susceptibility to lung diseases, such as lung cancer and chronic obstructive pulmonary disease, is largely influenced by the metabolic capacity of lung tissues. This capacity is partly determined by the expression profile of the cytochromes P450 (CYPs), a superfamily of enzymes that have relevant ca...

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Bibliographic Details
Published in:Biochimie 2010-03, Vol.92 (3), p.292-306
Main Authors: Leclerc, Julie, Tournel, Gilles, Courcot-Ngoubo Ngangue, Elisabeth, Pottier, Nicolas, Lafitte, Jean-Jacques, Jaillard, Sophie, Mensier, Eric, Lhermitte, Michel, Broly, Franck, Lo-Guidice, Jean-Marc
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Non-Small-Cell Lung - enzymology
Carcinoma, Non-Small-Cell Lung - genetics
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Cytochrome P450
Female
Gene expression
Gene Expression Profiling
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Lung
Lung - anatomy & histology
Lung - enzymology
Lung - pathology
Lung - physiology
Lung Neoplasms - enzymology
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Male
Microarray Analysis
Middle Aged
Non-small cell lung cancer
Reverse Transcriptase Polymerase Chain Reaction
TaqMan low-density array
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Summary:Susceptibility to lung diseases, such as lung cancer and chronic obstructive pulmonary disease, is largely influenced by the metabolic capacity of lung tissues. This capacity is partly determined by the expression profile of the cytochromes P450 (CYPs), a superfamily of enzymes that have relevant catalytic properties toward exogenous and endogenous compounds. Using quantitative real-time RT-PCR, we conducted a comprehensive analysis of the expression profile of the 57 human CYP genes in non-tumoral (bronchial mucosa and pulmonary parenchyma) and tumoral lung tissues of 18 patients with non-small cell lung cancer. This study highlights (i) inter-individual variations in lung expression for some CYPs, (ii) different CYP expression patterns between bronchial mucosa and pulmonary parenchyma, that indicate distinctive susceptibility of these tissues toward the deleterious effects of inhaled chemical toxicants and carcinogens, (iii) high intertumoral variability, that could have major implications on lung tumor response to anti-cancer drugs.
ISSN:0300-9084
1638-6183
DOI:10.1016/j.biochi.2009.12.007