Volatile organic compounds of Clavispora lusitaniae AgL21 restrain citrus postharvest pathogens

•C. lusitaniae AgL21 released antifungal VOCs against citrus postharvest pathogens.•Biofumigation by VOCs reduced severity of P. digitatum on postharvest lemons.•The yeast produced mostly isoamyl acetate and two alcohols as antifungals.•Isoamyl acetate inhibited fungistatically P. digitatum in vitro...

Full description

Saved in:
Bibliographic Details
Published in:Biological control 2022-11, Vol.174, p.105025, Article 105025
Main Authors: Pereyra, Martina María, Garmendia, Gabriela, Rossini, Carmen, Meinhardt, Friedhelm, Vero, Silvana, Dib, Julián Rafael
Format: Article
Language:English
Subjects:
Biological control
Clavispora lusitaniae
Lemons
Postharvest disease
Volatile organic compounds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•C. lusitaniae AgL21 released antifungal VOCs against citrus postharvest pathogens.•Biofumigation by VOCs reduced severity of P. digitatum on postharvest lemons.•The yeast produced mostly isoamyl acetate and two alcohols as antifungals.•Isoamyl acetate inhibited fungistatically P. digitatum in vitro.•3-methyl butanol and phenethyl alcohol had a fungicidal effect on the pathogen. Microbial agents exert their biocontrol effects through different mechanisms of action. Clavispora lusitaniae AgL21, a yeast capable of controlling postharvest diseases in lemon, produces volatile organic compounds (VOCs) impairing phytopathogenic fungi. Here we elucidate the nature of such compounds and check individual effects on mycelial growth of citrus fungal pathogens: Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii. VOCs characterization was carried out deploying solid-phase microextraction/gas chromatography-mass spectrum techniques. Among the compounds produced by C. lusitaniae AgL21, ethyl acetate, isoamyl acetate, 3-methyl butanol, and phenethyl alcohol displayed the highest relative abundance. Synthetic ethyl acetate, tested in vitro on P. digitatum, had no discernable effect on mycelial growth, whereas isoamyl acetate and both of the above alcohols exhibited fungistatic and fungicidal effects, respectively. The data provided in this study prove for the first time, that VOCs released by C. lusitaniae AgL21 are able to take part in antagonistic activities against citrus postharvest fungal pathogens.
ISSN:1049-9644
1090-2112
DOI:10.1016/j.biocontrol.2022.105025