Possible involvement of protein kinase C activation in differentiation of human umbilical vein endothelium-derived cell into smooth muscle-like cell

We previously reported that when deprived of fibroblast growth factor, human umbilical vein endothelium-derived cells (HUVE-DCs) are capable of differentiating into smooth muscle-like cells through activin A-induced, Smad-dependent signaling, and that maintenance of the endothelial-cell phenotype an...

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Bibliographic Details
Published in:Biology of the cell 2004-09, Vol.96 (7), p.499-499
Main Authors: Ishisaki, Akira, Tsunobuchi, Hironaka, Nakajima, Keiichi, Imamura, Toru
Format: Article
Language:English
Subjects:
Carbazoles - pharmacology
Carcinogens - pharmacology
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cells, Cultured
Endothelium, Vascular - physiology
Endothelium-derived cell
Enzyme Activation - drug effects
Enzyme Inhibitors - pharmacokinetics
Fibroblast growth factor
Humans
Indoles - pharmacology
Maleimides - pharmacology
Myocytes, Smooth Muscle - physiology
Protein kinase C
Protein Kinase C - antagonists & inhibitors
Protein Kinase C - metabolism
Signal Transduction - drug effects
Smooth muscle-like cell
Tetradecanoylphorbol Acetate - pharmacology
Umbilical Veins - cytology
Umbilical Veins - physiology
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Summary:We previously reported that when deprived of fibroblast growth factor, human umbilical vein endothelium-derived cells (HUVE-DCs) are capable of differentiating into smooth muscle-like cells through activin A-induced, Smad-dependent signaling, and that maintenance of the endothelial-cell phenotype and differentiation into smooth muscle-like cells are reciprocally controlled by fibroblast growth factor-1 and activin A (Ishisaki et al., 2003). Here, we examined how protein kinase C (PKC), which plays pivotal roles in the regulation of cellular proliferation and differentiation in numerous cell types, might affect the above differentiation. We found that phorbol-12-myristate-13-acetate-induced down-regulations of some PKCs accompany suppressions of the expressions of smooth muscle cell markers in HUVE-DCs deprived of fibroblast growth factor. Moreover, the PKC-inhibitors Gö6850 and Gö6983 suppressed the differentiation of HUVE-DCs into smooth muscle-like cells. These results strongly suggest that activation of PKC is involved in the above differentiation.
ISSN:0248-4900
1768-322X
DOI:10.1016/j.biolcel.2004.04.012