Effects of heparinoids from algae on hemostasis and their action on the cycle cell

• Published in: Biomedicine & preventive nutrition 2012-07, Vol.2 (3), p.163-168
• Main Authors: Alves, Monique Gabriela das Chagas Faustino, Nobre, Leonardo Thiago Duarte Barreto, Monteiro, Norberto de Kássio Vieira, Moura, Gioconda Emanuella Diniz de Dantas, Dore, Celina Maria Pinto Guerra, de Medeiros, Valquíria Pereira, Leite, Edda Lisboa
• Format: Article
• Language: English
• Subjects: Anticoagulant; Antithrombotic; Heparin-like compound; Hypnea musciformis; Seaweed
• ISSN: 2210-5239; 2210-5239
• DOI: 10.1016/j.bionut.2012.03.005

Heparin was the first component used as an anticoagulant and antithrombotic agent; therefore, its use presents limitations due to side effects, limited source of material and occurrence of contamination. Marine sulfated polysaccharides provide a low level of contamination by prions and viruses as they are extracted exclusively from a marine source. Heparin-like compounds, sulfated galactans, extracted from red marine algae Hypnea musciformis and fractionated with 1 volume of acetone (F1.0), were evaluated for their anticoagulant activity by activated partial thromboplastin time (aPTT) and prothrombin time (PT) tests by examining their action on enzymatic activity of thrombin and FXa, and their stimulatory action on antithrombotic heparan sulfate synthesis using [35S]-sodium sulfate, and determining how they promote the inhibition of cell proliferation. The aPTT test displayed a low anticoagulant activity at 350μg of F1.0 (∼90s) P