Evaluation radioprotective effect of curcumin conjugated albumin nanoparticles

• Published in: Bioorganic chemistry 2020-07, Vol.100, p.103891, Article 103891
• Main Authors: Nosrati, Hamed, Danafar, Hossein, Rezaeejam, Hamed, Gholipour, Nazila, Rahimi-Nasrabadi, Mehdi
• Format: Article
• Language: English
• Subjects: Animals; BSA; Cattle; Cell Line; Cell Survival - drug effects; Cell Survival - radiation effects; Curcumin; Curcumin - administration & dosage; Curcumin - chemistry; Curcumin - pharmacology; Drug Carriers - chemistry; Drug conjugate; Hemolysis - drug effects; Humans; Mice, Inbred BALB C; Nanoparticles - chemistry; Prodrugs - administration & dosage; Prodrugs - chemistry; Prodrugs - pharmacology; Radiation-Protective Agents - administration & dosage; Radiation-Protective Agents - chemistry; Radiation-Protective Agents - pharmacology; Radioprotection; Serum Albumin, Bovine - chemistry; X-Ray irradiation; X-Rays - adverse effects
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2020.103891

[Display omitted] •Preparation of Curcumin conjugated albumin nanoparticles (BSA-CUR) as radioprotection agent.•Incorporation of CUR into BSA was improved chemical stability of it.•Hazard Ratio (Mantel-Haenszel) of BSA-CUR treated groups are lower than control group.•The BSA-CUR can use as a proficient vehicle for improve potential radioprotective effect of CUR. In this research, curcumin (CUR) conjugated albumin based nanoparticles (BSA-CUR) were designed for improvement and evaluation radioprotective effect of CUR. In this way, we have prepared BSA-CUR by covalently binding the CUR with BSA. Next, this synthesized prodrug was evaluated for physical and chemical properties by Fourier-transform infrared (FTIR), Dynamic light scattering (DLS), Transmission electron microscopy (TEM), Ultraviolet–visible (UV/Vis), and Differential scanning calorimetry (DSC) analysis. Furthermore, the chemical stability of designed prodrug was appraised. The result shows that the size of nanoparticles is 174.4 nm with a polydispersity index (PdI) of 0.191. The nanoparticles have a high loading capacity and show sustained release behavior. Loading of CUR to BSA not only could increase the chemical stability of CUR, but also could improve radioprotection efficacy of it's against X-Ray irradiation. The HHF-2 cells show 107% viability in the presence of BSA-CUR at a concentration of 50 µg/mL, whereas non-treated cells show 46% viability, under X-Ray irradiation. Also in vivo study results show that, four out of five mice have died when the mice irradiated by X-Ray and no received any treatment. Although, for a group that treated with BSA-CUR and also irradiated by X-Ray, median survival and survival rate was higher than CUR treated and control mice, and only two out of five mice have died. The result of this study proved that BSA-CUR can be used as a proficient vehicle for improving the potential radioprotective effect of CUR.