5-Aza-2′-deoxycytidine increases the expression of anti-angiogenic vascular endothelial growth factor 189b variant in human lung microvascular endothelial cells

Abstract Vascular endothelial growth factor (VEGF) is involved in angiogenesis, growth, and tumour cell metastasis. VEGF is expressed as alternative splice variants, which exhibit angiogenic and anti-angiogenic properties. We determined the effect of 5-Aza-2′-deoxycytidine (5-dAzaC) DNA methyltransf...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2008-03, Vol.62 (3), p.158-163
Main Authors: Miller-Kasprzak, E, Jagodziński, P.P
Format: Article
Language:English
Subjects:
Angiogenesis
Antimetabolites, Antineoplastic - pharmacology
Azacitidine - analogs & derivatives
Azacitidine - pharmacology
Biological and medical sciences
Blotting, Western
Capillaries - cytology
Capillaries - drug effects
Cell Line
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Epigenetic regulation
Humans
Indicators and Reagents
Internal Medicine
Lung - drug effects
Lung - metabolism
Medical Education
Medical sciences
Pharmacology. Drug treatments
Reverse Transcriptase Polymerase Chain Reaction
Stimulation, Chemical
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - biosynthesis
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Summary:Abstract Vascular endothelial growth factor (VEGF) is involved in angiogenesis, growth, and tumour cell metastasis. VEGF is expressed as alternative splice variants, which exhibit angiogenic and anti-angiogenic properties. We determined the effect of 5-Aza-2′-deoxycytidine (5-dAzaC) DNA methyltransferase (DNMTs) inhibitor on angiogenic and anti-angiogenic VEGF variants expression in immortalized human lung microvascular endothelial cells (HLMEC). Employing reverse transcription, real-time quantitative PCR (RQ-PCR), and Western blot analysis, we determined that 5-dAzaC decreased VEGF121a and VEGF165a angiogenic, and VEGF121b and VEGF165b anti-angiogenic variants expression in HLMEC. However, this DNMTs inhibitor significantly increases expression of VEGF189b anti-angiogenic variant transcript and protein in HLMEC. Our results suggest that the DNMTs activity may have an influence on the expression of angiogenic and anti-angiogenic VEGF variants in human lung microvascular endothelial cells.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2007.07.015