Increased CXCR4 expression in AsPC1 pancreatic carcinoma cells with RNA interference-mediated knockdown of DNMT1 and DNMT3B

• Published in: Biomedicine & pharmacotherapy 2010-04, Vol.64 (4), p.254-258
• Main Authors: Przybylski, M, Kozłowska, A, Pietkiewicz, P.P, Lutkowska, A, Lianeri, M, Jagodzinski, P.P
• Format: Article
• Language: English
• Subjects: Azacitidine - analogs & derivatives; Azacitidine - pharmacology; Biological and medical sciences; Cell Line, Tumor; CXCR4; Decitabine; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferases - genetics; DNA Methylation; DNA Methyltransferase 3B; DNA methyltransferases; DNA, Neoplasm - genetics; Flow Cytometry; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation, Neoplastic; Humans; Internal Medicine; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Medical Education; Medical sciences; Pancreatic cancer; Pancreatic Neoplasms - genetics; Pharmacology. Drug treatments; Promoter Regions, Genetic; Receptors, CXCR4 - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Sequence Analysis, DNA; Tumors
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2009.06.008

Abstract The effect of DNA methylation on CXCR4 expression has been demonstrated in pancreatic cancer and melanoma cells, but little is known about the effect of DNA methyltransferases 1 and 3 (DNMT1 and DNMT3B) on CXCR4 expression. Employing lentiviral vectors, we created stable RNA interference-mediated knockdown of DNMT1 and DNMT3B in AsPC1 pancreatic cancer cells. Using reverse transcription real-time quantitative PCR and flow cytometric analysis, we evaluated the increase in the expression of CXCR4 transcript and protein levels in these cells. Bisulfite sequencing analysis showed that the level of promoter demethylation appeared more effective in cells with knockdown of DNMT1 than in those with DNMT3B knockdown. Furthermore, the combined RNA interference knockdown of both DNMT1 and DNMT3B increased promoter demethylation, leading to a slight increase in CXCR4 expression. However, the demethylating agent 5-Aza-2’-deoxycytidine exhibited the strongest effect on promoter demethylation, which correlated with the highest production of CXCR4 transcript and protein in AsPC1 cells. Our results indicate that DNMT1 plays the main role in maintenance of methylation of CXCR4 promoter, while DNMT3B may function as an accessory DNA methyltransferase to modulate CXCR4 expression in AsPC1 cells.