Silymarin induces a multi-targeted cell death process in the human colon cancer cell line HT-29

This study investigated the Silymarin (SM) effects on growth of HT-29 human colon cancer cell line and its cellular death mechanism. HT-29 cells were treated by 25μM/ml of SM for 48h. HT-29 cells were also pretreated with 10mmol zVAD (apoptosis inhibitor), 10mmol 3-MA (autophagy inhibitor) and 3mmol...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2017-10, Vol.94, p.890-897
Main Authors: Khorsandi, L., Saki, G., Bavarsad, N., Mombeini, M.
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - pharmacology
Apoptosis
Apoptosis - drug effects
Autophagy
Autophagy - drug effects
Caspases - metabolism
Cell Death - drug effects
Cell Proliferation - drug effects
Cell Survival - drug effects
Colon cancer
Colonic Neoplasms - drug therapy
HT29 Cells
Humans
Necroptosis
Receptor-Interacting Protein Serine-Threonine Kinases - genetics
Silymarin
Silymarin - pharmacology
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Summary:This study investigated the Silymarin (SM) effects on growth of HT-29 human colon cancer cell line and its cellular death mechanism. HT-29 cells were treated by 25μM/ml of SM for 48h. HT-29 cells were also pretreated with 10mmol zVAD (apoptosis inhibitor), 10mmol 3-MA (autophagy inhibitor) and 3mmol Nec (necroptosis inhibitor) for evaluation cell death induced by apoptosis, outophagy and necroptosis. MTT and clonogenicity assays revealed that the SM without inhibitors induced a significant decrease in cell viability and proliferation of HT-29 cells (p
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2017.08.015