Simultaneous quantification of Myelin Basic Protein and Tau proteins in cerebrospinal fluid and serum of Multiple Sclerosis patients using nanoimmunosensor

• Published in: Biosensors & bioelectronics 2017-03, Vol.89 (Pt 2), p.781-788
• Main Authors: Derkus, Burak, Acar Bozkurt, Pinar, Tulu, Metin, Emregul, Kaan C., Yucesan, Canan, Emregul, Emel
• Format: Article
• Language: English
• Subjects: Antibodies, Immobilized - chemistry; Biosensing Techniques - methods; Cadmium Compounds - chemistry; Dendrimer; Dendrimers - chemistry; Dielectric Spectroscopy - methods; Differential pulse voltammetry; Electrochemical impedance spectroscopy; Electrochemical Techniques - methods; Graphene oxide; Graphite - chemistry; Humans; Immunoassay - methods; Immunosensor; Lead - chemistry; Limit of Detection; Multiple Sclerosis; Multiple Sclerosis - blood; Multiple Sclerosis - cerebrospinal fluid; Myelin Basic Protein; Myelin Basic Protein - blood; Myelin Basic Protein - cerebrospinal fluid; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Oxides - chemistry; Sulfides - chemistry; Tau; tau Proteins - blood; tau Proteins - cerebrospinal fluid
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.10.019

This study was aimed at the development of an immunosensor for the simultaneous quantification of Myelin Basic Protein (MBP) and Tau proteins in cerebrospinal fluid (CSF) and serum, obtained from Multiple Sclerosis (MS) patients. The newly developed GO/pPG/anti-MBP/anti-Tau nanoimmunosensor has been established by immobilization of MBP and Tau antibodies. The newly developed nanoimmunosensor was tested, optimized and characterized using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The developed nanoimmunosensor was seen to have detection limits of 0.30nM for MBP and 0.15nM for Tau proteins which were sufficient for the levels to be analysed in neuro-clinic. The clinical study performed using CSF and serum of MS patients showed that the designed nanoimmunosensor was capable of detecting the proteins properly, that were essentially proven by ELISA. •A graphene oxide-dendrimer nanoplatform was prepared for antibody immobilization.•Antibody conjugated dendrimer-PbS or CdS electrochemical nanoprobs show excellent performance.•The developed nanoimmunosensor has detection limits of 0.30nM for MBP and 0.15nM for Tau proteins.