Electrochemical H2O2 biosensor composed of myoglobin on MoS2 nanoparticle-graphene oxide hybrid structure

• Published in: Biosensors & bioelectronics 2017-07, Vol.93, p.14-20
• Main Authors: Yoon, Jinho, Lee, Taek, Bapurao G., Bharate, Jo, Jinhee, Oh, Byung-Keun, Choi, Jeong-Woo
• Format: Article
• Language: English
• Subjects: Biosensor; Electrochemical signal enhancement; Graphene oxide; Hydrogen peroxide; MoS2 nanoparticle; Myoglobin
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.11.064

In this research, the electrochemical biosensor composed of myoglobin (Mb) on molybdenum disulfide nanoparticles (MoS2 NP) encapsulated with graphene oxide (GO) was fabricated for the detection of hydrogen peroxide (H2O2). Hybrid structure composed of MoS2 NP and GO (GO@MoS2) was fabricated for the first time to enhance the electrochemical signal of the biosensor. As a sensing material, Mb was introduced to fabricate the biosensor for H2O2 detection. Formation and immobilization of GO@MoS2 was confirmed by transmission electron microscopy, ultraviolet-visible spectroscopy, scanning electron microscopy, and scanning tunneling microscopy. Immobilization of Mb, and electrochemical property of biosensor were investigated by cyclic voltammetry and amperometric i-t measurements. Fabricated biosensor showed the electrochemical signal enhanced redox current as −1.86μA at an oxidation potential and 1.95μA at a reduction potential that were enhanced relative to those of electrode prepared without GO@MoS2. Also, this biosensor showed the reproducibility of electrochemical signal, and retained the property until 9 days from fabrication. Upon addition of H2O2, the biosensor showed enhanced amperometric response current with selectivity relative to that of the biosensor prepared without GO@MoS2. This novel hybrid material-based biosensor can suggest a milestone in the development of a highly sensitive detecting platform for biosensor fabrication with highly sensitive detection of target molecules other than H2O2. •The electrochemical biosensor composed of the myoglobin on the graphene oxide (GO)-encapsulated molybdenum disulfide nanoparticles (MoS2 NP) was developed for hydrogen peroxide (H2O2) detection.•MoS2 NP were encapsulated by GO (GO@MoS2) for the first time to induce the electrochemical signal enhancement.•Fabricated electrochemical biosensor with GO@MoS2 showed the stability and reproducibility.•Fabricated electrochemical biosensor also showed the enhanced amperometric response current by detection of H2O2.