Isolation, characterization and optimizations of laccase producer from soil: A comprehensive study of application of statistical approach to enhance laccase productivity in Myrothecium verrucaria NFCCI 4363

• Published in: Bioresource technology reports 2021-09, Vol.15, p.100751, Article 100751
• Main Authors: Jawale, J.P., Nandre, V.S., Latpate, R.V., Kulkarni, M.V., Doshi, P.J.
• Format: Article
• Language: English
• Subjects: Box-Behnken Design; Dye decolorization; Laccase; Myrothecium verrucaria; Plackett-Burman Design
• ISSN: 2589-014X; 2589-014X
• DOI: 10.1016/j.biteb.2021.100751

The aim of this work was to find the optimal cultivation conditions of Myrothecium verrucaria NFCCI 4363 to enhance its laccase productivity. Interactive effect of twelve physicochemical parameters were determined and optimized by Plackett-Burman Design (PBD) and Box-Behnken Design (BBD). After statistical experimental performance, significant factors were determined and confirmed as yeast extract, 0.3%; temperature, 25 °C; ABTS, 0.5 mM; MgSO4, 1.5 mM and CuSO4, 0.5 mM. Laccase productivity of M. verrucaria NFCCI 4363 in optimized media was 1415.77 ± 42.34 U/L, more two times increased than unoptimized condition. Stable and optimal conditions of the produced laccase was determined as pH 5, 20 °C and pH 6, 50 °C, respectively. Therefore by statistical approach optimal values of cultural parameters were achieved. Moreover, crude laccase showed more than 70% decolorization potential of bromothymol blue, bromocresol green and bromocresol purple dyes in absence of any of the mediators. [Display omitted] •M. verrucaria NFCCI 4363 was confirmed for laccase production.•Enhanced laccase productivity was obtained by statistical methods.•Produced laccase was active at pH 6, 50 °C, and tolerate to Cd2+, Cu2+, Fe2+ ions.•Extracellular laccase decolorized dyes without mediators.