Biotechnological potential of a new strain of Cylindrotheca fusiformis producing fatty acids and fucoxanthin

• Published in: Bioresource technology reports 2022-06, Vol.18, p.101098, Article 101098
• Main Authors: Gevorgiz, Ruslan G., Gontcharov, Andrey A., Zheleznova, Svetlana N., Malakhova, Ludmila V., Alyomova, Tatiana E., Maoka, Takashi, Nekhoroshev, Mikhail V.
• Format: Article
• Language: English
• Subjects: Fucoxanthin; Growth rate; Marine microalgae; Polyunsaturated fatty acids
• ISSN: 2589-014X; 2589-014X
• DOI: 10.1016/j.biteb.2022.101098

A new strain of benthic diatom Cylindrotheca fusiformis producing fucoxanthin (Fx) and polyunsaturated fatty acids (PUFAs) proved to be promising for biotechnology. Identification of Fx was carried out from the analysis of the ultraviolet and visible (UV-VIS) absorption spectra, electrospray ionization mass spectra (ESI MS) and proton magnetic resonance spectra (1H NMR). The specific growth rate of the culture was 0.52 1/day, and the maximum productivity 1.56 g/(L × day). The accumulation rates of eicosapentaenoic and arachidonic acids in the stationary growth phase were 4.5 and 3.2 mg/(L × day), respectively, and that of Fx was 6 mg/(L × day). The accumulation of PUFAs in the biomass reached 4.68% of dry weight. The Fx content in the biomass reached 21.4 mg/g. The strain settles to the photobioreactor bottom in the absence of mixing, which property simplifies the biomass harvesting. [Display omitted] •The fucoxanthin accumulation rate in the C. fusiformis strain is 6 mg/L day−1.•Polyunsaturated fatty acids (46.8%) dominate with an ω6/ω3 ratio of 0.96.•The strain is benthic and quickly precipitates, which reduces the harvesting costs.