Cu 2+ -labeled dansyl compounds as fluorescent and PET probes for imaging apoptosis

Compound DNSTT-Cu , a novel chelate of Cu with DOTA conjugated to a fluorescent dansyl fragment, is developed for imaging cell apoptosis. Apoptotic U-87MG cells could be selectively visualized by the fluorescence of DNSTT-Cu from cytoplasm of cells, confirmed by the fluorescence of apoptosis cells c...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2016-11, Vol.26 (22), p.5594-5596
Main Authors: Han, Junyan, Wang, Xukui, Yu, MeiXiang
Format: Article
Language:English
Subjects:
Annexin A5 - analysis
Apoptosis
Cations, Divalent - chemistry
Cell Line, Tumor
Chelating Agents - chemistry
Copper - chemistry
Copper Radioisotopes - chemistry
Dansyl Compounds - chemistry
Fluorescent Dyes - chemistry
Heterocyclic Compounds, 1-Ring - chemistry
Humans
Microscopy, Confocal - methods
Optical Imaging - methods
Positron-Emission Tomography - methods
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Summary:Compound DNSTT-Cu , a novel chelate of Cu with DOTA conjugated to a fluorescent dansyl fragment, is developed for imaging cell apoptosis. Apoptotic U-87MG cells could be selectively visualized by the fluorescence of DNSTT-Cu from cytoplasm of cells, confirmed by the fluorescence of apoptosis cells co-labeled with Alexa Fluor 568-labeled annexin V, a conventional probe for selectively labeling membranes of apoptosis cells. A radioactive Cu analog, DNSTT- Cu , was easily synthesized, providing a potential PET probe for imaging apoptosis in vivo.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2016.09.057