Lipopolysaccharides and peptidoglycans modulating the interaction of Au naparticles with cell membranes models at the air-water interface

• Published in: Biophysical chemistry 2018-07, Vol.238, p.22-29
• Main Authors: da Silva, Rafael Leonardo Cruz Gomes, da Silva, Heloiza Fernanda Oliveira, da Silva Gasparotto, Luiz Henrique, Caseli, Luciano
• Format: Article
• Language: English
• Subjects: Airwater interface; Cell membrane models; Gold nanoparticles; Monolayers
• ISSN: 0301-4622; 1873-4200
• DOI: 10.1016/j.bpc.2018.04.007

Understanding the interactions between nanoparticles and biological surfaces is of great importance for many areas of nanomedicine and calls for detailed studies at the molecular level using simplified models of cellular membranes. In this paper, water-dispersed polyvinylpyrrolidonestabilized gold nanoparticles (AuNPs) were incorporated in floating monolayers of selected lipids at the air-water interface as cell membrane models. Surface pressure-area isotherms showed the condensation of glycoside-free lipid monolayers, suggesting their adsorption on the nanoparticle surface through the hydrophilic head groups. On the other hand, monolayers containing glycoside derivatives expanded upon AuNPs incorporation, pointing that the supramolecular structure formed should facilitate the incorporation of these nanoparticles in cellular membranes. These findings can be therefore correlated with the possible toxicity, microbicide and antitumorigenic effects of these nanoparticles in lipidic surfaces of erythrocyte and microbial membranes. [Display omitted] •Au nanoparticles were stabilized with PVP.•They were incorporated in membrane models.•Surface properties of the films was affected.•Monolayer composition modulates interaction.