Apelin-36 mediates neuroprotective effects by regulating oxidative stress, autophagy and apoptosis in MPTP-induced Parkinson’s disease model mice

• Published in: Brain research 2020-01, Vol.1726, p.146493, Article 146493
• Main Authors: Zhu, Junge, Gao, Wenming, Shan, Xuehua, Wang, Chunmei, Wang, Huiqing, Shao, Ziqi, Dou, Shanshan, Jiang, Yunlu, Wang, Chuangong, Cheng, Baohua
• Format: Article
• Language: English
• Subjects: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridin; Animals; Apelin - administration & dosage; Apelin - metabolism; Apelin-36; Autophagy; Autophagy - drug effects; Corpus Striatum - drug effects; Corpus Striatum - metabolism; Disease Models, Animal; Dopamine - metabolism; Gene Expression Regulation - drug effects; Male; Mice, Inbred C57BL; MPTP Poisoning - metabolism; Neuroprotective Agents - administration & dosage; Nitrated α-synuclein; Oxidative stress; Oxidative Stress - drug effects; Parkinson’s disease; Pars Compacta - drug effects; Pars Compacta - metabolism; Signal Transduction - drug effects
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2019.146493

•Apelin-36 protected against MPTP-induced dopamine depletion in the STR of mice.•Apelin-36 reduced oxidative stress and iNOS expression in MPTP-treated mice.•Apelin-36 reversed MPTP-induced nitrated α-synuclein expression in mice.•Apelin-36 alleviated the impaired autophagy induced by MPTP of mice.•Apelin-36 inhibited ASK1/JNK/caspase-3 apoptotic pathway in mice. Parkinson’s disease (PD), a common human neurodegenerative disorder, is characterized by the presence of intraneuronal Lewy bodies composed principally of abnormal aggregated and post-translationally modified α-synuclein. In our previous research, we have demonstrated the neuroprotective effect of Apelin-36, a neuroendocrine peptide in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP)-lesioned PD model mice. Therefore, this study was designed to evaluate the neuroprotective mechanism of Apelin-36 against MPTP-induced neurotoxicity in mice. The results showed that MPTP-induced the depletion of dopamine in the striatum (STR) was partially reversed by Apelin-36. Apelin-36 also improved the activity of antioxidant system including superoxide dismutase (SOD) and glutathione (GSH), and decreased the overproduction of malondialdehyde (MDA) in the substantia nigra pars compacta (SNpc) and STR of MPTP-treated mice. Moreover, Apelin-36 downregulated inducible nitric oxide synthase (iNOS) and nitrated α-synuclein expression. Furthermore, Apelin-36 significantly promoted autophagy indicated by the up-regulation of LC3-II and Beclin1 and inhibition of p62 expression in the SNpc and STR of MPTP-treated mice. The protective effect of Apelin-36 was also associated with the inhibition of the apoptosis signal‐regulating kinase 1 (ASK1)/c‐Jun N‐terminal kinase (JNK) signaling pathway and inactivation of caspase-3. Taken together, our findings demonstrated that the neuroprotective mechanism of Apelin-36 against MPTP-induced neurotoxicity in mice might be related to decreasing the aggregation of nitrated α-synuclein and alleviating oxidative stress as well as promoting autophagy and inhibiting ASK1/JNK/caspase-3 apoptotic pathway, which provides a novel strategy for PD treatment.