Phytochemical and chemotaxonomic study of Pulsatilla cernua (Thunb.) Bercht. ex J. Presl

• Published in: Biochemical systematics and ecology 2021-08, Vol.97, p.104291, Article 104291
• Main Authors: Yan, Yahui, Zhang, Lingxiao, Liu, Xin, Zhu, Qiang, Wang, Yu, Liang, Hongyue, Chen, Zaixing, Yang, Yanyun, Xu, Liang, Liu, Tao
• Format: Article
• Language: English
• Subjects: Chemotaxonomy; MS analysis; NMR analysis; Pulsatilla cernua (Thunb.) Bercht. ex J. presl; Ranunculaceae
• ISSN: 0305-1978; 1873-2925
• DOI: 10.1016/j.bse.2021.104291

Twenty-two compounds were isolated from the 70% EtOH–H2O extract of Pulsatilla cernua (Thunb.) Bercht. ex J. Presl roots, and their structures were determined based on 1H NMR, 13C NMR and MS spectroscopic data, including (+)-pinoresinol (1), matairesinol (2), 4-ethoxycinnamic acid (3), p-hydroxy ethyl cinnamate (4), 3-(4′-methoxyphenyl)-2(E)-propenoic acid (5), methyl 4-hydroxycinnamate (6), radicol (7), cryptomeridiol (8), fraxinellone (9), diolmycin B2 (10), hederagonic acid (11), hederagenin (12), oleanolic acid (13), 3-O-α-L-arabinopyranosyl-oleanolic acid (14), hederagenin 3-O-α-L-arabinopyranoside (15), 3-O-[α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl] oleanolic acid (16), hederasaponin B (17), kizutasaponin K12 (18), patrinia saponin H3 (19), hederacholichiside F (20), cernuoside A (21) and cernuoside B (22). Eight compounds (3–10) were isolated and identified from the genus Pulsatilla for the first time. •Twenty-two compounds were isolated from the roots of Pulsatilla cernua (Thunb.) Bercht. ex J. Presl.•Compounds 3–10 were isolated from the genus Pulsatilla for the first time.•Limonoid (compound 9) was isolated from the family Ranunculaceae for the first time.•Compound 10 was isolated from plant resources for the first time.•The significance of these compounds in the chemotaxonomy of the genus was discussed.