Spondias purpurea Exudate polysaccharide as affinity matrix for the isolation of a galactose-binding-lectin

• Published in: Carbohydrate polymers 2007-11, Vol.70 (4), p.369-377
• Main Authors: Teixeira, Daniele M.A., Braga, Renata C., Horta, Ana C.G., Moreira, Renato A., de Brito, Ana C.F., Maciel, Jeanny S., Feitosa, Judith P.A., de Paula, Regina C.M.
• Format: Article
• Language: English
• Subjects: Affinity chromatography; Applied sciences; Artocarpus altilis; Artocarpus heterophyllus; binding properties; Biological and medical sciences; chemical composition; Chemical constitution; chemical structure; Erythrina; Erythrina velutina; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; gels; hemagglutination; Lectins; Natural polymers; Physicochemistry of polymers; plant exudates; Plant physiology and development; Polysaccharide; polysaccharides; Ricinus communis; shrubs; Spondias purpurea; Starch and polysaccharides; trees
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2007.04.016

Spondias purpurea L., popularly known as ciriguela, is native and widespread tree from Mexico through Northern Peru and Brazil, particularly in semi-arid zones. This tree exudes a water soluble polysaccharide, constituted of a (1→3) linked galactan backbone substituted at C6 with d-galactose, d-xylose, l-arabinose, l-rhamnose and glucuronic acid units. Brazilian polysaccharide differs from Venezuelan on the amount of acid and arabinose as well as the presence of fucose and glucose as minor sugar. The d-galactose substitution (1→6) confers to the polysaccharide the peculiar capacity of binding α- d-galactose specific lectins after cross-linking with epichlorohydrin. The gel obtained was able to specifically retain d-galactose-binding-lectins, among with those from Artocarpus incisa, Artocarpus integrifolia, Erythrina velutina and Ricinus communis. On the other hand, no glucose-binding-lectins were retained.