A polysaccharide isolated from Agaricus blazei Murill inhibits sialyl Lewis X/E-selectin-mediated metastatic potential in HT-29 cells through down-regulating α-1,3-fucosyltransferase-VII (FucT-VII)

• Published in: Carbohydrate polymers 2010-03, Vol.79 (4), p.921-926
• Main Authors: Liu, Jicheng, Yue, Liling, Zhang, Chun, Fan, Li, Zhou, Li, Lin, Yu, Niu, Yingcai, Li, Xueyan, Wen, Xianchun, Sun, Yongxu
• Format: Article
• Language: English
• Subjects: Adhesion; Agaricus blazei; Antineoplastic agents; Applied sciences; Biological and medical sciences; E-selectin; Exact sciences and technology; General aspects; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Sialyl Lewis X; Starch and polysaccharides; α-1,3-Fucosyltransferase-VII
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2009.10.023

In this research, one low molecular weight polysaccharide (LMW-ABP) was isolated from the fruiting bodies of Agaricus blazei Murill. We reported that the effect of LMW-ABP on inhibiting the interaction between E-selectin and sialyl Lewis X (sLe x) by flow cytometry and real-time reverse transcription polymerase chain reaction (RT-PCR) technology. Different concentrations of LMW-ABP (5, 10, and 20 mg/L) could effectively dose-dependently inhibit adhesion of HT-29 cells to human umbilical vein endothelial cells (HUVECs) in static conditions, as well as down-regulating the expression of both α-1,3-fucosyltransferase-VII (FucT-VII) and sLe x in transcription or translation level, respectively. These results suggest that LMW-ABP could suppress the metastasizing capacity of cancer cells through interfering with the interaction between E-selectin and sLe x. Thus based on these findings, LMW-ABP is expected to be having enormous potential for use in treatment for neoplasm metastasis.