In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligo-chitosan (O-C), N, O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC)...

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Bibliographic Details
Published in:Carbohydrate polymers 2010-03, Vol.79 (4), p.1094-1100
Main Authors: Abdull Rasad, Mohammad Syaiful Bahari, Halim, Ahmad Sukari, Hashim, Kamaruddin, Rashid, Ahmad Hazri Abdul, Yusof, Norimah, Shamsuddin, Shaharum
Format: Article
Language:English
Subjects:
Applied sciences
Chitosan derivatives
Cytocompatibility
Exact sciences and technology
Hypertrophic scars
Matrix metalloproteinase-13
Natural polymers
Paste
Physicochemistry of polymers
Primary human fibroblasts
Sheet
Starch and polysaccharides
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Summary:The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligo-chitosan (O-C), N, O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2009.10.048