Water-soluble polysaccharide from the fruiting bodies of Chroogomphis rutilus (Schaeff.: Fr.) O. K. Miller: Isolation, structural features and its scavenging effect on hydroxyl radical

• Published in: Carbohydrate polymers 2010-05, Vol.80 (3), p.720-724
• Main Authors: Sun, Yongxu, Li, Xia, Yang, Jingfeng, Liu, Jicheng, Kennedy, John F.
• Format: Article
• Language: English
• Subjects: Anti-oxidant activity; Applied sciences; Biological and medical sciences; Chroogomphis rutilus; Exact sciences and technology; General and cellular metabolism. Vitamins; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Starch and polysaccharides; Structural analysis
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2009.12.015

One water-soluble polysaccharide (CRP) was isolated from the fruiting bodies of Chroogomphis rutilus with a molecular weight ( M w) of 3.2 × 10 4 Da. According to partial acid hydrolysis, periodate oxidation and Smith degradation, methylation, FT–IR, GC–MS, and NMR analysis, the results indicated CRP had a backbone consisting of (1→6)-linked-α- d-galactopyranosyl and (1→2, 6)-linked-α- d-galactopyranosyl residues that terminated in a single terminal (1→)-β- d-glucopyranosyl residue at the O-2 position of (1→2, 6)-linked-α- d-galactopyranosyl residue along the main chain in the ratio of 1:1:1. Furthermore, the in vitro anti-oxidant activity evaluated by hydroxyl radicals scavenging method showed that CRP could remarkably enhance the scavenging effect on hydroxyl radicals in a dose-dependent manner.