Isolation and structural characterization of a novel galactomannan from Eremurus anisopterus (Ker. et Kir) Regel roots

A novel galactomannan was obtained from the roots of Eremurus anisopterus (Ker. et Kir) Regel, an ephemeroid plant in the Gurbantunggut Desert in Xinjiang. The crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation w...

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Bibliographic Details
Published in:Carbohydrate polymers 2011-02, Vol.84 (1), p.402-406
Main Authors: Hu, Changfeng, Kong, Qingjun, Yang, Deyu, Pan, Yuanjiang
Format: Article
Language:English
Subjects:
anion exchange chromatography
Applied sciences
Biological and medical sciences
centrifugation
Chemical constitution
Eremurus
Eremurus anisopterus
ethanol
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Galactomannan
galactomannans
gel chromatography
Gel filtration
methylation
molecular weight
Natural polymers
nuclear magnetic resonance spectroscopy
oxidation
Physicochemistry of polymers
Plant physiology and development
Polysaccharide
roots
Starch and polysaccharides
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Summary:A novel galactomannan was obtained from the roots of Eremurus anisopterus (Ker. et Kir) Regel, an ephemeroid plant in the Gurbantunggut Desert in Xinjiang. The crude polysaccharide was extracted following the usual separation procedure, including water dissolution, centrifugation and precipitation with ethanol. The crude polysaccharide was then applied to anion-exchange chromatography and gel filtration to obtain a homogeneous polysaccharide E1. The average molecular weight of E1 was estimated to be 6kDa according to the calibration curve. On the basis of composition analysis, methylation analysis, periodate oxidation analysis, Smith degradation, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, E1 is a novel linear galactomannan with a main chain composed of (1→6)-linked α-d-Galp and (1→2)-α-d-Manp at a relative molar ratio of 1:3.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2010.11.054